Analysis Note

ControlSkin (basement membrane), High grade ovarian tumors

Application

Research Sub CategoryIntegrins

Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.

Research CategoryCell Structure

Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)

ELISA/RIA: 1:1000

Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit

Immunoprecipitation: 5 µL precipitates alpha2/beta1 from 5 x 106 cells.

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Neat rabbit antiSerum. Liquid with 0.05% sodium azide

Unpurified

Specificity

Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

160kDa